In vitro assessment of toxic effects of a commercial formulation ammonium salt of glyphosate over five species representing different trophic levels and habitats

Authors

  • Martín Spósito Servicio de Evaluación y Control de la Calidad Ambiental. Intendencia de Montevideo (SECCA)
  • Julio Cesar Espínola Moltedo Dr. en Medicina y Tecnologías Veterinarias, del Servicio de Evaluación y Control de la Calidad Ambiental. Intendencia de Montevideo (SECCA)

DOI:

https://doi.org/10.26461/12.05

Keywords:

TryGlif, Herbicide, Bioassays, Ecotoxicity, Superficial waters

Abstract

We evaluated the acute and chronic toxic effect in vitro of a commercial brand of ammonium salt of gliphosate over five species that occupy different trophic levels and physical environments: bacterioplankton (Vibrio fischeri), phytoplankton (Pseudokirchneriella subcapitata), zooplankton (Daphnia magna), zoobenthos (Hydra attenuata) and terrestrial (Lactuca sativa). This investigation assesed the commercial preparation of herbicide Try Glif, based on ammonium salt of glyphosate with a concentration of 430gr/L by weight of glyphosate, and inert compounds (dispersants, emulsifiers, solubilizers, etc.) with a potential toxicity is generally synergistic with the active principle. Among the five species tested the most sensitive to the compound was H. attenuata, with an LC50 of 5,79 mg/L. The luminescent bacterium V. fischeri was from the five bioassays that a median effective concentration scored higher, reaching 37,28 mg / L. The unicellular alga P. subcapitata had an LC50 of 24,58 mg/L. The D. magna and L. sativa had similar values of LC50 of 9,59 mg/L and 9,79mg/L respectively. Quantified effects on different species analyzed provide a baseline for further investigations and were compared to those found in similar experiments in the international literature

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References

Asociación Española de Normalización y Certificación, 1996. UNE-ISO 6341: Calidad de agua. Determinación de la inhibición de la movilidad de Daphnia magna Straus (Cladocera, Crustacea). Ensayo de toxicidad aguda. Madrid: AENOR.

Alberdi, J.L., Sáenz, M.E., Di Mazio, W.D., Tortorelli, M.C., 1996. Comparative acute toxicity of two herbicides, paraquat and glyphosate, to Daphnia magna and D.spinulata. En: Bulletin of Environmental Contamination and Toxicology, 57, pp.229–235.

Aparicio, V.C., et al., 2013. Environmental fate of glyphosate and aminomethylphosphonic acid in surface waters and soil of agricultural basins. En: Chemosphere, 93(9), pp.1866-1873.

Bonnet, J.L., Bonnemoy, F., Dusser, M. y Bohatier, J., 2007. Assessment of the potential toxicity of herbicides and their degradation products to non target cells using two microorganisms, the bacteria Vibriof ischeri and the ciliate. En: Tetrahymena pyriformis, Environ. Toxicol.,22(1), pp.78-91.

De Roos, A. J., Zahm, S., Cantor, K., Weisenburger, D., Holmes, F., Burmeister, L. y Blair, A., 2003. Integrative assessment of multiple pesticides as risk factors for non-Hodgkin’s lymphoma among men. En: Occupational and Environmental Medicine, 60(9), e11.

Dutka, B.J., 1989. Methods for microbiological and toxicological analysis of waters, wastewaters, and sediments. Ontario: Ed. Rivers Research Branch, NWRI, CCIW, Burlington.

Eagleson, K.W., Lenat, D.L., Ausley, L.W. y Winborne, F.B., 1990. Comparison of measured instream biological responses with responses predicted using the Ceriodaphnia dubia chronic toxicity test. En: Environ. Toxicol. Chem., (9), pp.1019–1028.

Environment Canada, 2007. Guidance document in statistical methods for environmental toxicity tests. Ottawa: Environment Canada. (Report EPS 1/RM/46).

Folmar, L.C., Sanders, H.L. y Julin, A.M., 1979. Toxicity of the herbicide glyphosate and several of its formulations to fish and aquatic invertebrates. En: Arch. Environ. Contam. To x i c o l, 8(3), pp.269-278.

Guyton, K.Z., Loomis, D., Grosse, Y., El Ghissassi, F., Benbrahim-Tallaa, L., Guha, N., Scoccianti, C., Mattock, H. y Straif, K., 2015. Carcinogenicity of tetrachlorvinphos, parathion, malathion, diazinon, and glyphosate. En: Lancet Oncol, 16(5), pp.490-1.

Johnson, E.M., Gabel, B.E.G, Newman, L.M. y Giacobbe, R., 1990. The Hydra assay manual. A practical guide to supplies, techniques and mechanics of the assay. Filadelfia: Department of Anatomy, Daniel Baugh Institute, Jefferson Medical College.

Lallana, María del C., et al., 2013. Determinación de reducción del crecimiento radical (CE50) por una formulación de glifosato utilizando lechuga y trigo como especies bioindicadoras [En línea]. En: Rev. Fac. Cienc. Agrar., Univ. Nac. Cuyo, 45(1). [Consulta: enero de 2015].Disponible en: http://revista.fca.uncu.edu.ar/images/stories/pdfs/2013-01/T45_01_13_Lallana.pdf

Martin, M.L., Sobrero, M.C., Rivas, C., Rimoldi, F. y Ronco, A., 2003. Impacto del uso de pesticidas asociado a la siembra directa sobre especies no-blanco: flora riparia y acuática. En: Memorias de la Conferencia Internacional Usos Múltiples del Agua. Cartagena de Indias: Universidad del Valle.

Martin, M.L. y Ronco, A.E., 2006. Effects of mixtures of pesticides used in the direct seedling technique on non-target plant seeds. En: Bulletin of Environmental Contamination and Toxicology, 77(2), pp.228-236.

Martino, D. L., 1995. El herbicida glifosato: su manejo más allá de la dosis por hectárea. Montevideo: INIA. (Serie Técnica, no. 61).

Mensah, P.K., Muller, W.J., y Palmer, C.G., 2011. Acute toxicity of Roundup (R) herbicide to three life stages of the freshwater shrimp Caridina nilotica (Decapoda: Atyidae). En: Phys Chem Earth, 36, pp.905–909.

Mensah, P.K., Palmer, G. y Muller, Wilhelmine J., 2014. Using growth measures in the freshwater shrimp Caridina nilotica as biomarkers of Roundup® pollution of South African freshwater systems. En: Phys Chem Earth, (50–52), pp.262–268.

Mensah, Paul, K., C., Palmer, G. y Wilhelmine J. Muller, 2014. Lethal and sublethal effects of pesticides on aquatic organisms: the case of a freshwater shrimp exposure to Roundup® [En línea]. Soloneski, Sonia ed. Pesticides - toxic aspects. [s.l.]: InTech. ISBN: 978-953-51-1217-4. DOI: 10.5772/57166. [Consulta: noviembre de 2015]. Disponible en: http://www.intechopen.com/books/pesticides-toxic-aspects/lethal-and-sublethal-effects-of-pesticides-on-aquatic-organisms-the-case-of-a-freshwater-shrimp-expo.

Norberg-King, T.J., 1993. A linear interpolation method for sublethal toxicity: the inhibition concentration (ICP) approach. Minnesota: US EPA. (Technical report 03–93).

Nuñez, S., Maeso, D., Franchi, S., Fiorentino G., Chouhy A., Ferrazzini, H., Fernandez A., Takahashi, S. y Gonda, G., 2012. Evaluación del escurrimiento superficial de plaguicidas en suelos agrícolas representativos del país. Montevideo: INIA, JICA, mgAP

Pérez, G.P., Vera, M.S. y Miranda, L., 2011. Effects of herbicide glyphosate and glyphosate-based formulations on aquatic ecosystems [En línea]. En: Kortekamp, A. ed., Herbicides and environment. [s.l.]: InTech. ISBN 978-953-307-476-4. DOI: 10.5772/12877. [Consulta: noviembre de 2015]. Disponible en: http://www.intechopen.com/books/herbicides-and-environment/effects-of-herbicide-glyphosate-and-glyphosate-based-formulations-on-aquatic-ecosystems

Peruzzo, P., Marino, D., Cremonte, C., da Silva, M., Porta, A. y Ronco, A., 2003. Impacto de pesticidas en aguas superficiales y sedimento asociado a cultivos por siembra directa. Memorias Conferencia Internacional Usos del Agua, Cartagena de Indias. Cartagena de Indias: IWA. pp.135-142.

Peruzzo, P.J., Porta, A.A., Ronco, A.E., 2008. Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina. En: Environ. Pollut., 156(1), pp.61-66.

REDES, 2012. Crecimiento de la agricultura y el uso de agrotóxicos en Uruguay [En línea]. Montevideo: Redes Amigos de la Tierra – Uruguay. [Consulta: noviembre de 2015]. Disponible en: http://www.redes.org.uy/wp-content/uploads/2014/01/Folleto-Agr-y-Agrotoxicos-Redes-WEB.pdf.

Relyea, R. A., 2005. The impact of insecticides and herbicides on the biodiversity and productivity of aquatic communities. En: Ecol. Appl., 15(2), pp.618-627.

Sobrero, M.C., Rimoldi, F., Ronco, A.E., 2007. Effects of the glyphosate active ingredient and a formulation on Lemma gibba L. at different exoposure levels and assessment end-points. En: Bull. Environ. Contam. Toxicol.,79, pp.537–543.

Trottier, S., Blaise C., T. Kusui y E.M. Johnson, 1997. Acute toxicity Assessment of Aqueous Samples Using a Microplate-based Hydra attenuata Assay. En: Environmental Toxicology and Water Quality, 12, pp.265-271.

U.S. Department of Agriculture Economic Research Service, USDA ERS, 2013. Adoption of genetically engineered crops in the U.S. Washington, DC. [En línea]. Washington: USDA ERS. [Consulta: octubre de 2015]. Disponible en: www.ers.usda.gov/data-products/ adoption-of genetically-engineered-crops-in-the-us.aspx#. UeRCQW0fhIQ

US EPA, 1993. R.E.D. Facts: glyphosate. Washington: US EPA, p.7.

US EPA, 1996. Ecological effects test guidelines. Seed germination/root elongation toxicity test. Minnesota: US EPA. (OPPTS 850.4200).

van Gestel, C. A. M., van der Waarde, J. J., Derksen, J. G. M., van der Hoek, E. E., Veul, M. F. X. W., Bouwens, S., Rusch,B., Kronenburg, R., y Stokman, G. N. M., 2001. The use of acute and chronic bioassays to determine the ecological risk and bioremediation efficiency of oil-polluted soils. En: Environ. Toxicol. Chem, 20, pp.1438–1449.

Published

2016-12-06

How to Cite

Spósito, M., & Espínola Moltedo, J. C. (2016). In vitro assessment of toxic effects of a commercial formulation ammonium salt of glyphosate over five species representing different trophic levels and habitats. INNOTEC, (12 ago-dic), 48–53. https://doi.org/10.26461/12.05

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