Three-dimensional scaffolds Sponges type based in bioconjugates collagen-chitosan as a potential biomaterial for tissue engineering applications

Authors

  • Mikol Ramos Escuela de Química, Universidad Nacional, Costa Rica
  • Vanessa Zamora Mora Universidad Nacional de Costa Rica. Laboratorio de Polímeros. Costa Rica
  • Gerardo Rodríguez Escuela de Química, Universidad Nacional, Costa Rica
  • María del Rosario Sibaja Ballestero Escuela de Química, Universidad Nacional, Costa Rica
  • Sergio Madrigal Carballo Escuela de Química, Universidad Nacional, Costa Rica
  • Mary Isabel Lopretti Correa Facultad de Ciencias, UdelaR, Uruguay

DOI:

https://doi.org/10.26461/07.07

Keywords:

Chitosan, colagen, scaffolds 3D, engineering tissue

Abstract

We isolated chitosan from native shrimp waste streams and collagen from tilapia skin waste; composite materials were successfully obtained by mixing chitosan with collagen (80:20 v/v). Chitosan-collagen composites were formulated as a 3D sponge-like scaffold, applying previously developed methodologies involving solvent casting and freeze drying. Chitosan-collagen 3D scaffolds were characterized according to its thermal behavior (TG) and morphology (SEM). Finally, we study the effect of the addition of a porogenic agent on the water absorption capacity and morphology of the 3D scaffolds.

Downloads

Download data is not yet available.

References

BERTHOD, F.; SAINTIGNY, G.; CHRETIEN, F.; HAYEK, D.; COLLOMBEL, C.; DAMOUR, O. Optimization of thickness, pore size and mechanical properties of a biomaterial designed for deep burn coverage. En: Clin. Mater. 1994, 15:259-65.

BERTHOD, F.; HAYEK, D.; DAMOUR, O.; COLLOMBEL, C. Collagen synthesis by human fibroblasts cultured within a collagen sponge. En: Biomaterials. 1993, 14:749-54.

CAO, Y.; CROLL, T.I.; LEES, J.G.; TUCH, B.E.; COOPER-WHITE, J.J. Scaffolds, stem cells and tissue engineering: A potent combination. En: Chem. Inform. 2006, 37:15–21.

CHANDY, T.; SHARMA, C.P. Chitosan matrix for oral sustained delivery of ampicillin En: Biomaterials. 1993, 14:939–944.

CHANDY, T.; SHARMA, C.P. Chitosan: as a biomaterial. En: Biomater. Artif. Cell Artif. Org. 1990, 18:1–24.

CHEN, G.; SATO, T.; OHGUSHI, H.; USHIDA, T.; TATEISHI, T.; TANAKA, J. Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh. En: Biomaterials. 2005, 26:2559-66.

CIMA, L.G.; VACANTI, J.P.; INGBER, D.; MOONEY, D.; LANGER, R. Tissue engineering by cell transplantation using degradable polymer substitutes. En: J. Biomech. Eng. 1991, 113:143–151.

HIRANO, S. Chitin biotechnology applications. En: Biotechnol Annu. Rev. 1996, 2:237–258.


HOLLINGER, J.O.; BATTISON, G.C. Biodegradable bone repair materials: synthetic polymers and ceramics. En: Clin. Orthop. 1986, 207:290–305.

LAURENCIN, C.T.; EL-AMIN, S.F.; IBIM, S.E.; WILOUGH, D.A.; ATTAWIA, M.; ALLOCK, H.R. AMBROCIO, A.A. A highly porous 3-dimensional polyphosphazene polymer matrix for skeletal tissue regeneration. En: J. Biomed. Mater. Res. 1996, 30:133–138.

LEE, CH.; SINGLA, A.; LEE, Y. Biomedical applications of collagen. En: Int. J. Pharm. 2001, 221:1-22.

MA, J.B.; WANG, H.J.; HE, B.L.; CHEN, J.T. A preliminary in vitro study on the fabrication and tissue engineering applications of a novel chitosan bilayer material as a scaffold of human neofetal dermal fibroblasts. En: Biomaterials. 2001, 22:331–6.

MAO, J.; ZHAO, L.; YAO, K.; SHANG, Q.; YANG, G.; CAO, Y. Study of novel chitosan–gelatin artificial skin in vitro. En: J Biomed Mater Res A. 2003, 64:301–8.

MUZARELLI, R.A.A. Biochemical significance of exogenous chitins and chitosans in animals and patients. En: Carbohydr. Polym. 1993 20:7–16.

MUZZARELLI, R.A.A.; ZUCCHINI, C.; ILARI, P.; PUGNALONI, A.; MATTIOLI-BELMONTE, M.; BIAGINI, G. Stimulatory effect on bone formation exerted by a modified chitosan. En: Biomaterials. 1993, 14:925–929.

NAZAROV, R.; JIN, H.J.; KAPLAN, D.L. Porous 3D scaffolds from regenerated silk fibroin. En: Biomacromolecules. 2004, 5:718–726.

OJEH, N.O.; FRAME, J.D.; NAVSARIA, H.A. In vitro characterization of an artificial dermal scaffold. En: Tissue Eng. 2001, 7:457-72.

OKAMOTO, Y.; SHIBAZAKI, K.; MINAMI, S.; MATSUHASHI, A.; TANIOKA, S.; SHIGEMASA, Y. Evaluation of chitin and chitosan on open wound healing in dogs. En: J Vet Med Sci. 1995, 57:851–4.

PACHENCE, J.M. Collagen-based devices for soft tissue repair. En: J Biomed Mater Res. 1996, 33:35-40.

PARK, S.N.; LEE, H.J.; LEE, K.H.; SUH, H. Biological characterization of EDC crosslinked collagen–hyaluronic acid matrix in dermal tissue restoration. En: Biomaterials. 2003, 24:1631-41.

PATEL, V.R.; AMIJI, M.M. Preparation and characterization of freeze-dried chitosan–poly(ethylene oxide) hydrogels for site-specific antibiotic delivery in the stomach. En: Pharm. Res. 1996, 13:588–593.

PIANIGIANI, E.; ANDREASSI, A.; TADDEUCCI, P.; ALESSANDRINI, C.; FIMIANI, M. ANDREASSI, L. A new model for studying differentiation and growth of epidermal cultures on hyaluronan-based carrier. En: Biomaterials. 1999, 20:1689–94.

VASUDEV, S.C.; CHANDY, T.; SHARMA, C.P. Development of chitosan–polyethylene vinyl acetate co-matrix: controlled release of aspirin–heparin for preventing cardiovascular thrombosis. En: Biomaterials. 1997, 18:375–381.

YANG, Y.; ZHOU, Y.; TAN, W.S. Effect of additives on collagen sponges for bioactive artificial skin. En: J Funct Polym. 2004, 17:396–400.

YAYLAOGLU, M.B.; KORKUSUZ, P.; ORS, U.; KORKUSUZ, F.; HASIRCI, V. Development of calcium phosphate–gelatin composite as a bone substitute and its use in drug release. En: Biomaterials. 1999, 20:711–719.

YOU, Y.; PARK, W.H.; KO, B.M.; MIN, B.M. Effects of PVA sponge containing chitooligosaccharide in the early stage of wound healing. En: J Mater Sci Mater Med. 2004, 15:297–301.

ZAMORA, V. Elaboración de apósitos biológicos a partir de colágeno extraído de pieles de tilapia. Heredia: Universidad Nacional de Costa Rica, 2008. (Tesis de grado).

Published

2012-12-16

How to Cite

Ramos, M., Zamora Mora, V., Rodríguez, G., Sibaja Ballestero, M. del R., Madrigal Carballo, S., & Lopretti Correa, M. I. (2012). Three-dimensional scaffolds Sponges type based in bioconjugates collagen-chitosan as a potential biomaterial for tissue engineering applications. INNOTEC, (7 ene-dic), 43–48. https://doi.org/10.26461/07.07

Issue

Section

Articles