Obtención de leche bovina reducida en grasa saturada y naturalmente enriquecida en ácido linoleico conjugado

Autores/as

DOI:

https://doi.org/10.26461/15.01

Palabras clave:

Vaca lechera, alimentación estabulada, perfl de la grasa láctea

Resumen

El objetivo del trabajo fue reducir la presencia de los ácidos grasos (AG) saturados potencialmente aterogénicos (C12:0, C14:0 y C16:0) de la leche y aumentar la presencia del ácido linoleico conjugado (CLA) en vacas lecheras estabuladas alimentadas con ración completamente mezclada (RCM) a través del aporte suplementario de ácidos grasos poliinsaturados (AGPI). Se utilizaron dos lotes de 70 vacas multíparas en el primer tercio de lactancia. Luego de seis semanas experimentales y durante cinco días consecutivos se obtuvieron cinco muestras de leche estándar (LE) provenientes del tanque colector del lote vacas sin AGPI suplementarios, y cinco muestras de leche CLA (LCLA) provenientes del tanque de las vacas suplementadas con los AGPI. El tenor graso (g/100g) de la LE (3,84) resultó superior (p<0,001) al de LCLA (2,84) por reducción (17,21%) de los AG potencialmente aterogénicos. El índice aterogénico fue mayor (p<0,001) en la LE (4,07) comparado al de la LCLA (2,58). La concentración del CLA aumentó (p<0,001) un 122% en LCLA (0,77 g/100 g) respecto al valor registrado en LE (0,34 g/100 g). El ácido oleico (g/100 g) resultó superior (p<0,001) en la LCLA (23,37) comparado con la LE (18,78). El aporte suplementario de AGPI permitió incrementar el valor saludable de la leche producida en un sistema de alimentación estabulado manteniendo una adecuada relación (6,59) omega-6/omega-3 en la leche y confrmando la plasticidad en la composición de los AG constitutivos de la grasa láctea.  

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AbuGhazaleh, A. y Jenkins, T. C., 2004. Short communication: decosahexaenoic acid promotes vaccenic acid accumulation in mixed rumen cultures when incubated with linoleic acid. En: Journal Dairy Sci., 87, pp.1047-1050.

AbuGhazaleh, A.A., Potu, R.B. y Ibrahim, S., 2009. Short communication: the effect of substituting fsh oil in dairy cow diets with docosahexaenoic acid-micro algae on milk composition and fatty acids profle. En: Journal of Dairy Science, 92(12), pp.6156–6159.

Antonacci, L.E., Gagliostro, G.A., Cano, A.V. y Bernal, C.A., 2017. Effects of feeding combinations of soybean and linseed oils on productive performance and milk fatty acid profle in grazing dairy cows. En: Agricultural Sciences, 8, pp. 984-1002. https://doi.org/10.4236/as.2017.89072

Awad, A.B., Hermann, T., Fink, C.S. y Horvath, P.J., 1995. 18:1 n7 Fatty acids inhibit growth and decreased inositol phosphate release in HT-29 cells compared to n-9 fatty acids. En: Cancer Lett., 91, pp.55-61.

Banni, S., Angioni, E., Murru, E., Carta, G., Melis, M.P., Bauman, D., Dong, Y. y Ip, C., 2001. Vaccenic acid feeding increases tissue levels of conjugated linoleic acid and suppreses development of premalignant lesions in rat mammary gland. En: Nutrition and Cancer, 41, pp.91-97. https://doi.org/10.1080/01635581.2001.9680617

Bargo, F., Muller, L.D., Kolver, E.S. y Delahoy, J.E., 2003. Invited review: production and digestion of supplemented dairy
cows on pasture. En: Journal of Dairy Science, 86, pp.1-42.

Bhattacharyaa, A., Banua,J., Rahmana, M., Causeyb J. y Fernandes, G., 2006. Biological effects of conjugated linoleic acids in health and disease. En: Journal of Nutritional Biochemistry, 17, pp.789–810.

Calder, P.C., 2006. n-3 Polyunsaturated fatty acids, inflammation, and inflammatory Diseases. En: American Journal of Clinical Nutrition, 83 (Suppl 1), pp.505S–19S.

Calder, P.C., 2008. Fatty acids and inflammation from the membrane to the nucleus and from the laboratory bench to the clinic. En: Clinical Nutrition, 2. pp.5–12.

Calder, P.C., 2012. Fatty acids and inflammatory processes: nutrition or pharmacology? En: British Journal of Clinical Pharmacology, 75 (3), pp.645–662.

Chilliard, Y., Ferlay, A., Mansbridge, R.M. y Doreau, M., 2000. Ruminant milk fat plasticity: nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids. En: Annales de Zootechnie, 49, pp.181-205. https://doi.org/10.1051/animres:2000117

Chilliard, Y. y Ferlay, A., 2004. Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties. En: Reprod. Nutr. Dev., 44, pp. 467–492.

Chilliard, Y., Glasser, G., Enjalbert, F., Ferlay, A., Bocquier, F. y Schimidely, P.H., 2007. Conferencia: resultados recientes
sobre los efectos de la alimentación en la composición en ácidos grasos de la leche de vaca, cabra y oveja. En: Revista
Argentina de Producción Animal, 27(3), pp.197-213.

Chilliard, Y., Martin, C., Rouel, J. y Doreau, M. 2009. Milk fatty acids in dairy cows fed whole crude linseed, extruded linseed, or linseed oil, and their relationship with methane output. En: Journal of Dairy Science, 92, pp.5199–5211.

Daviglus, M.L., Stamler, J., Orencia, A.J., Dyer, A.R., Liu, K., Greenland. P., Walsh, M.K., Morris, D. y Shekelle, R.B., 1997. Fish consumption and the 30-year risk of fatal myocardial infarction. En: New England Journal of Medicine, 336, pp.1046-1053. DOI: 10.1056/NEJM19970410336150.

Dhiman, T.R., Nam, S.H., Ure, AL., 2005. Factors affecting conjugated linoleic acid content in milk and meat. En: Crit. Rev. Food Sci. Nutr., 45, pp.463–82.

Gagliostro, G. A. y Chilliard, Y., 1992. Utilización de lípidos protegidos en la nutrición de vacas lecheras. Efectos sobre
la producción y composición de leche, y sobre la ingestión de materia seca y energía. En: Revista Argentina de Producción Animal, 12(1), pp.1-15.

Gagliostro, G.A., Antonacci, L., Espiñeira, M., Williner, M.R. y Bernal, C.A., 2013. Suplementación con ácidos grasos
poliinsaturados y perfl de ácidos grasos en vacas lecheras. En: Revista Argentina de Producción Animal, 33, (Supl. 1).

Gagliostro, G.A., Antonacci, L.E., Ballistreri, J., Bonina, E., Williner, M.R. y Bernal, C.A., 2012. Effects of a solid oil supplement (Oralac) on milk fatty acid composition of grazing dairy cows. En: Journal of Animal Science, 90, Suppl. 3/Journal of Dairy Science, 95, Suppl. 2., pp.119.

Gagliostro, G.A., Garciarena, D.A., Rodriguez, M.A. y Antonacci, L.E., 2017. Feeding polyunsaturated supplements to grazing dairy cows improve the healthy value of milk fatty acids. En: Agricultural Sciences, 8, pp.759-782. https://doi.org/10.4236/as.2017.88057.

Gillingham, L.G., Harris-Janz, S. y Jones, P.J., 2011. Dietary monounsaturated fatty acids are protective against metabolic syndrome and cardiovascular disease risk factors. En: Lipids, 46, pp.2019-228.

Glasser, F., Ferlay, A. y Chilliard, Y., 2008. Oilseed lipid supplements and fatty acid composition of cow milk: a Meta-Analysis. En: Journal of Dairy Science, 91, pp.4687-4703.

Griinari, J.M. y Bauman, D.E., 1999. Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. En: M. P. Yurawecz, M. M. Mossoba, J. K. G. Kramer, M. W. Pariza and G. J. Nelson, ed. Advances in conjugated linoleic acid research. Champaign: AOCS Press, Champaign. pp.180-200.

Grummer, R.R., 1991. Effect of feed on the composition of milk fat. En: Journal of Dairy Science, 74, pp.3244-3257.

Ha, Y.L., Storkson J. y Pariza, M.W., 1990. Inhibition of benzo(a) pyreneinduced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. En: Cancer Research, 50, pp.1097-1101.

Hodgson, J.M., Wahlqvist, M.L., Boxall, J.A. y Balazs, N.D., 1996. Platelet trans fatty acids in relation to angiographically assessed coronary artery disease. En: Atherosclerosis, 120, pp.147-154.

Houseknecht, K.L., Vanden Heuvel, J.P, Moya-Camarena, S.Y., et al., 1998. Dietary conjugated linoleic acid normalizes
impaired glucose tolerance in the Zucker diabetic fatty fa/ fa rat. En: Biochemical and Biophysical Research Commun,
244, pp.678–682.

Huang, Y., Schoonmaker, J.P., Bradford, B.J. y Beitz, D.C., 2008. Response of milk fatty acid composition to dietary
supplementation of soy oil, conjugated linoleic acid, or both. En: Journal of Dairy Science, 91, pp.260–270.

Ip, C., Banni, S., Angioni, E., Carta, G., McGinley, J., Tompson, H.J., Barbano, D. y Bauman, D., 1999. Conjugated linoleic acid-enriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats. En: J. Nutr., 129, pp.2135-2142.

Larson, S.C, Bergkvist, L. y Wolk, A., 2005. Hight-fat dairy food and conjugated linoleic acid intakes in relation to colorectal cancer incidence in the Swedish Mammography cohort. En: American Journal of Clinical Nutrition, 82, pp.894-900.

Lee, K.N., Kritchevsky, D. y Pariza, M.W., 1994. Conjugated linoleic acid and atherosclerosis in rabbits. En: Atherosclerosis, 108, pp.19-25.

Livingstone, K.M., Lovegrove, J.A. y Givens, D.I., 2012. The impact of substituting SFA in dairy products with MUFA
or PUFA on CVD risk: evidence from human intervention studies. En: Nutrition Research Reviews, 25(2), pp.193-206. doi:10.1017/S095442241200011X.

Masson, L.T., Alfaro, C., Camilo, A., Carvalho, P., Illesca, R., Torres, M., Tavares do Carmo, J. Mancini-Filho y Bernal,
C.A., 2015. Fatty acid composition of soybean/sunflower mix oil, fsh oil and butterfat applying the AOCS Ce 1j-07
method with a modifed temperature program. En: Grasas y Aceites, 66, pp.e064. ISSN-L: 0017-3495.
doi: http://dx.doi.org/10.3989/gya.0692141).

Mensink, R.P., Zock, P.L., Kester, A. D. y Katan, M.B., 2003. Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta analysis of 60 controlled trials. En: American Journal of Clinical Nutrition, 77, pp.1146-1155.

Miller, C.C., Park, Y., Pariza, M.W. y Cook, M.E., 1994. Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. En: Biochemical and Biophysical Research Communication, 198, pp.1107-1112.

Palmquist, D. L., Beaulieu, A. D. y Barbano, D. M., 1993. ADSA foundation symposium: Milk fat synthesis and modifcation. Feed and animal factors influencing milk fat composition. En: Journal of Dairy Science, 76, pp.1753-1771.

Palmquist, D. y Griinari, M., 2006. Milk fatty acid composition in response to reciprocal combinations of sunflower and fsh oils in the diet. En: Animal Feed Science and Technology, 131, pp.358–369.

Parodi, P.W., 2003. Conjugated linoleic acid in food. En: Sébédio, J.L., Christie, W.W., Adloff, R., eds. Advances in conjugated linoleic acid in food. Vol. 2. Illinois: AOCS Press. pp.101-122.

Piperova, L.L., Teter, B.B., Bruckental, I., Sampugna, J. Mills S.,E., Yurawecz,M.P., Fritsche, J., Ju, K. y Erdman, R.A., 2000. Mammary lipogenic enzyme activity, trans fatty acids and conjugated fatty acids are altered in lactating dairy cows fed a milk-fat depressing diet. En: Journal of
Nutrition, 130, pp.2568-2574.

Roy, A., Chardigny, J.M., Bauchart, D., Ferlay, A., Lorenz, S., Durand, D., Gruffat, D., Faulconnier, Y., Sébedio, J.M. y Chilliard, Y. 2007. Butters rich either in trans-10-C18:1 or in trans-11-C18:1 plus cis-9, trans-11 CLA differentially affect plasma lipids and aortic fatty streak in experimental atherosclerosis in rabbits. En: Animal, 1, pp.467-476.

Ryder, J.W., Portocarrero, C.P., Song, X.M, et al., 2001. Isomerspecifc antidiabetic properties of conjugated linoleic acid. Improved glucose tolerance, skeletal muscle insulin action, and UCP-2 gene expression. En: Diabetes, 50, pp.1149–1157.

Schrezenmeir, J. y Jagla, A., 2000. Milk and diabetes. En: Journal of the Animal College of Nutrition, 19(2), pp.176:190.

Schroeder, G.F., Gagliostro, G.A., Bargo, F., Delahoy, J.E. y Muller, L.D., 2004. Effects of fat supplementation on milk production and composition by dairy cows on pasture: a review. En: Livestock Production Science, 86(1-3), pp.1-18.

Siurana, A. y Calsamiglia, S., 2016. A metaanalysis of feeding strategies to increase the content of conjugated linoleic acid (CLA) in dairy cattle milk and the impact on daily human consumption. En: Animal Feed Science and Technology, 217, pp.13-26.

Smit, L.A., Baylin, A. y Campos, H., 2010. Conjugated linoleic acid in adipose tissue and risk of myocardial Infarction. En: American Journal of Clinical Nutrition, 92(1), pp.34-40.

Stanton, C., Murphy, J., McGrath, E. y Devery, R., 2003. Animal feeding strategies for conjugates linoleic acid enrichment of milk. En: Sébédio, J.L., Christie, W.W., Adloff, R., eds. Advances in Conjugated Linoleic Acid in Food. Vol 2. Illinois: AOCS Press. pp.123-145.

Turpeinen, A.M., Mutanen, M., Aro, A., Salminen, I., Basu, S., Palmquist, D.L. y Griinari, J.M., 2002. Bioconversion of vaccenic acid to conjugated linoleic acid in humans. En: American. Journal of Clinical Nutrition, 76, pp.504–510.

Ulbritch, T. L. V. y Southgate, D. A.T., 1991. Coronary heart disease: seven dietary factors. En: Lancet, 338, pp.985-992.

Watkins, B.A. y Li, Y., 2003. CLA in functional food: enrichment of animal products. En: Sébédio, J.L, Christie, W.W., Adloff, R., eds. En: Advances in conjugated linoleic acid in food. Vol 2. Illinois: AOCS Press. pp.174-188.

Wu Z. y Huber, J.T., 1994. Relationship between dietary fat supplementation and milk protein concentration in lactating cows: A review. En: Livestock Production Science, 39, pp.141-155.

Yang M. y Cook, M.E., 2003. Dietary conjugated linoleic acid decreased cachexia, macrophage tumor necrosis factoralpha production, and modifes splenocyte cytokines production. En: Experimental Biological Medicine, (Maywood), 228, pp.51-58.

Zheng, H.C., Liu, J.X., Yao, J.H., Yuan, Q., Ye, H.W., Ye, J.A. y Wu, Y.M., 2005. Effects of dietary sources of vegetable
oils on performance of high- yielding lactating cows and conjugated linoleic acids in milk. En: J. Dairy Sci., 88, pp.2037–2042.

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Publicado

2018-06-18

Cómo citar

Gagliostro, G. A., Antonacci, L. E., Carabajal, A., López Plavan, J. M., & Crujeira, Y. (2018). Obtención de leche bovina reducida en grasa saturada y naturalmente enriquecida en ácido linoleico conjugado. INNOTEC, (15 ene-jun), 37–43. https://doi.org/10.26461/15.01

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