Efecto de la formulación de galletas en la secreción de hormonas de saciedad


  • Nuria Martinez-Saez CIAL, Instituto de Investigación de Ciencias de la Alimentación (UAM-CSIC), Madrid, España
  • Christina Maria Hochkogler Departamento de Química Nutricional y Fisiológica, Facultad de Química, Universidad de Viena, Austria
  • Veronika Somoza Departamento de Química Nutricional y Fisiológica, Facultad de Química, Universidad de Viena, Austria
  • María Dolores del Castillo CIAL, UAM-CSIC. España



Palabras clave:

alimento con fines médicos, alto contenido en fibra, alimento saludable, bajo índice glicémico, calidad sensorial, fibra antioxidante de café, FOS, galletas, GLP-1, saciedad, serotonina, stevia


El objetivo de esta investigación fue desarrollar una nueva formulación de galleta con bajo índice glicémico y elevada calidad sensorial para el control efectivo de la saciedad. Se emplearon como ingredientes stevia (edulcorante hipocalórico), fructooligosacáridos (FOS) para mejorar la tolerancia a carbohidratos y calidad sensorial del alimento, y fibra dietética antioxidante de café (marros de café). Los alimentos e ingredientes individuales se digirieron in vitrosimulando condiciones de digestión humana. La liberación in vitro de péptido similar al glucagón tipo 1 (GLP1) y serotonina se estimuló incubando células humanas intestinales, HuTu-80 y Caco-2, con digeridos de galletas (0,5, 0,05 y 0,01 mg/ml) durante 90 min y 5 min, respectivamente. La cuantificación de las hormonas GLP1 y serotonina se realizó por ELISA. La secreción de hormonas en células tratadas con digeridos de galletas tradicional y nueva fueron del mismo orden de magnitud (p>0,05). La fibra antioxidante del café estimuló la secreción de hormonas saciantes mientras que la stevia resultó inefectiva. La sustitución de sacarosa por stevia no afectó la capacidad saciante de las galletas. En conclusión, se ha logrado un nuevo alimento con alto contenido en fibra antioxidante, saludable, saciante, de buena calidad sensorial apto para individuos sanos y diabéticos.


Los datos de descargas todavía no están disponibles.


Anton, S, Martin, C, Han, H, Coulon, S, Cefalu, W, Geiselman, P y Williamson, D, 2010. Effects of stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels. En: Appetite, 55(1), pp.37–43.

Bakuradze, T, Parra, G, Riedel, A, Somoza, V, Lang, R, Dieminger, N y Richling, E, 2014. Four-week coffee consumption affects energy intake, satiety regulation, body fat, and protects DNA integrity. En: Food Research International, 63, pp.420-427.

Choi, S, DiSilvio, B, Fernstrom, M y Fernstrom, J, 2009. Meal ingestion, amino acids and brain neurotransmitters: Effects of dietary protein source on serotonin and catecholamine synthesis rates. En: Physiology and Behavior, 98(1-2), pp.156–162.

Choi, S, DiSilvio, B, Fernstrom, M y Fernstrom, J, 2011. The chronic ingestion of diets containing different proteins produces marked variations in brain tryptophan levels and serotonin synthesis in the rat. En: Neurochemical Research, 36(3), pp.559–565.

del Castillo, M., Martinez-Saez, N. y Ullate, M., 2014. Healthy bakery products with high level of dietary antioxidant fibre. Patente española. PCT/ES2014/070062.

Delzenne, N, Cani, P y Neyrinck, A, 2007. Modulation of Glucagon-like Peptide 1 and Energy Metabolism by Inulin and Oligofructose: Experimental Data. En: Journal of Nutrition, 137(11), pp.2547S–2551S.

Edwards, A y Slater, N, 2009. Protection of live bacteria from bile acid toxicity using bile acid adsorbing resins. En: Vaccine, 27(29), pp.3897–3903.

EFSA, 2014. Scientific Opinion on the substantiation of a health claim related to non-digestible carbohydrates and a reduction of post-prandial glycaemic responses pursuant to Article 13 (5) of Regulation (EC) No 1924/2006. En: EFSA Journal, 12(1), pp.1–13.

Frost, G, Brynes, A, Dhillo, W, Bloom, S y McBurney, M, 2003. The effects of fiber enrichment of pasta and fat content on gastric emptying, GLP-1, glucose, and insulin responses to a meal. En: European Journal of Clinical Nutrition, 57, pp.293–298.

Fujii, Y, Osaki, N, Hase, T y Shimotoyodome, A, 2015. Ingestion of coffee polyphenols increases postprandial release of the active glucagon-like peptide-1 (GLP-1(7–36)) amide in C57BL/6J mice. En: Journal of Nutritional Science, 4, (e9), pp.1-9.

Fujita, Y, Wideman, R, Speck, M, Asadi, A, King, D, Webber, T y Kieffer, T, 2009. Incretin release from gut is acutely enhanced by sugar but not by sweeteners in vivo. En: American Journal of Physiology. Endocrinology and Metabolism, 296(3), pp.E473–E479.

Garcia-Serna, E, Martinez-Saez, N, Mesias, M, Morales, F, y del Castillo, M, 2014. Use of coffee silverskin and stevia to improve the formulation of biscuits. En: Polish Journal of Food and Nutrition Sciences, 64(4), pp.243–251.

Geraedts, M, Troost, F, Fischer, M, Edens, L y Saris, W, 2011. Direct induction of CCK and GLP-1 release from murine endocrine cells by intact dietary proteins. En: Molecular Nutrition and Food Research, 55(3), pp.476–484.

Geraedts, M., Troost, F, Tinnemans, R, Söderholm, J, Brummer, R y Saris, W, 2010. Release of satiety hormones in response to specific dietary proteins is different between human and murine small intestinal mucosa. En: Annals of Nutrition and Metabolism, 56(4), pp.308–313.

Gostner, J, Schroecksnadel, S, Jenny, Klein, A, Ueberall, F, Schennach, H y Fuchs, D, 2015. Coffee Extracts Suppress Tryptophan Breakdown in Mitogen-Stimulated Peripheral Blood Mononuclear Cells. En: Journal of the American College of Nutrition, 34(3), pp.212–223.

Grover, G, Koetzner, L, Wicks, J, Gahler, R, Lyon, M, Reimer, R y Wood, S, 2011. Effects of the soluble fiber complex PolyGlycopleX® (PGX®) on glycemic control, insulin secretion, and GLP-1 levels in Zucker diabetic rats. En: Life Sciences, 88(9-10), pp.392–399.

Habib, N, Honoré, S, Genta, S y Sánchez, S, 2011. Hypolipidemic effect of Smallanthus sonchifolius (yacon) roots on diabetic rats: Biochemical approach. En: Chemico-Biological Interactions, 194(1), pp.31–39.

Hellfritsch, C, Brockhoff, A, Stähler, F, Meyerhof, W y Hofmann, T, 2012. Human psychometric and taste receptor responses to steviol glycosides. En: Journal of Agricultural and Food Chemistry, 60(27), pp.6782–6793.

Herrera, C, Smith, K, Atkinson, F, Ruell, P, Chow, C, O’Connor, H y Brand-Miller, J, 2011. High-glycaemic index and -glycaemic load meals increase the availability of tryptophan in healthy volunteers. En: The British Journal of Nutrition, 105(11), pp.1601–1606.

Holik, A, Rohm, B, Somoza, M y Somoza, V, 2013. N(ε)-Carboxymethyllysine (CML), a Maillard reaction product, stimulates serotonin release and activates the receptor for advanced glycation end products (RAGE) in SH-SY5Y cells. En: Food and Function, 4(7), pp.1111–20.

Hollebeeck, S, Borlon, F, Schneider, Y, Larondelle, Y y Rogez, H, 2013. Development of a standardised human in vitrodigestion protocol based on macronutrient digestion using response surface methodology. En: Food Chemistry, 138(2-3), pp.1936–1944.

Huang, W, Chen, Z, Wang, Q, Lin, M, Wu, S, Yan, Q y Pan, J, 2013. Piperine potentiates the antidepressant-like effect of trans-resveratrol: Involvement of monoaminergic system. En: Metabolic Brain Disease, 28(4), pp.585–595.

Ishwarya, S, y Prabhasankar, P, 2013. Fructooligosaccharide – Retention during baking and its influence on biscuit quality. En: Food Bioscience, 4, pp.68–80.

Jokura, H, Watanabe, I, Umeda, M, Hase, T y Shimotoyodome, A, 2015. Coffee polyphenol consumption improves postprandial hyperglycemia associated with impaired vascular endothelial function in healthy male adults. En: Nutrition Research, 35(10), pp.873–881.

Kumar, V, Sinha, A, Makkar, H, de Boeck, G y Becker, K, 2012. Dietary Roles of Non-Starch Polysachharides in Human Nutrition: A Review. En: Critical Reviews in Food Science and Nutrition, 52(10), pp.899–935.

Lee, C. Y, 2016. Glucagon-like Peptide-1 Formulation - the Present and Future Development in Diabetes Treatment. En: Basic and Clinical Pharmacology and Toxicology, 118, pp.173–180.

Li, P, Lin, W, Wang, Y, Peng, W, Cai, X y Su, W, 2012. Antidiabetic activities of oligosaccharides of Ophiopogonis japonicus in experimental type 2 diabetic rats. En: International Journal of Biological Macromolecules, 51(5), pp.749–755.

Liu, Y, Lan, N, Ren, J, Wu, Y, Wang, S-T, Huang, X y Yu, Y, 2015. Orientin improves depression-like behavior and BDNF in chronic stressed mice. En: Molecular Nutrition and Food Research, 59, pp.1130–1142.

Manning, P, Sutherland, W, Manning, A, de Jong, S y Berry, E, 2013. Ingestion of thermally oxidized sunflower oil decreases postprandial lipemia mainly in younger individuals. En: Nutrition Research, 33(9), pp.711–718.

Mawe, G y Hoffman, J, 2013. Serotonin Signaling in the Gastrointestinal Tract: Functions, dysfunctions, and therapeutic targets. En: Nature Reviews Gastroenterology and Hepatology, 10(8), pp.473–486.

McCarty, M, 2005. A chlorogenic acid-induced increase in GLP-1 production may mediate the impact of heavy coffee consumption on diabetes risk. En: Medical Hypotheses, 64(4), pp.848–853.

Nutritional Heart, Lung, and Blood Institute, 2013. Managing overweight and obesity in adults: systematic evidence review from the obesity expert panel. Washington: U.S Department of Health and Human Services.

Rafferty, E, Wylie, A, Elliott, C, Chevallier, O, Grieve, D y Green, B, 2011. In vitro and in vivo effects of natural putative secretagogues of Glucagon-like peptide-1 (GLP-1). En: Scientia Pharmaceutica, 79(3), pp.615–621.

Unión Europea. Reglamento (CE) 1924/2006, de 20 de diciembre de 2006. Official Journal of the European Union, 30 de diciembre de 2006, No. L404/9.

Sartori da Silva, A, Haas, P, Sartori, N, Anton, A y Francisco, A, 2007. Fructoligosaccharides: active dietary fibers. En: Boletim Do Centro de Pesquisa E Processamento de Alimentos, 25(2), pp.295–304.

Sharma, R, Yadav, R, y Manivannan, E, 2012. Study of effect of Stevia rebaudiana bertoni on oxidative stress in type-2 diabetic rat models. En: Biomedicine and Aging Pathology, 2(3), pp.126–131.

Tamanna, N y Mahmood, N, 2015. Food processing and maillard reaction products: effect on human health and nutrition. En: International Journal of Food Science, 2015, pp.1–6.

Van Bloemendaal, L, IJzerman, R, Ten Kulve, J, Barkhof, F, Konrad, R, Drent, M y Diamant, M, 2014. GLP-1 receptor activation modulates appetite- and reward-related brain areas in humans. En: Diabetes, 63(12), pp.4186–4196.

Voigt, J y Fink, H, 2015. Serotonin controlling feeding and satiety. En: Behavioural Brain Research, 277, pp.14–31.

World Health Organization, 2014. Global satus report on noncommunicable diseases 2014 [En línea]. Geneva: WHO. [Consulta: 14 de abril de 2016] Disponible en: http://www.who.int/mediacentre/factsheets/fs311/es/#.

Zelkas, L, Raghupathi, R, Lumsden, A., Martin, A, Sun, E, Spencer, N y Keating, D, 2015. Serotonin-secreting enteroendocrine cells respond via diverse mechanisms to acute and chronic changes in glucose availability. En: Nutrition and Metabolism, 12(1), pp.55.




Cómo citar

Martinez-Saez, N., Hochkogler, C. M., Somoza, V., & del Castillo, M. D. (2016). Efecto de la formulación de galletas en la secreción de hormonas de saciedad. INNOTEC, 1(11 ene-jul), 65–72. https://doi.org/10.26461/11.09




Artículos más leídos del mismo autor/a