Purification and Characterization of a lectin from Amaranthus hypochondriacus, an antiproliferative compound

Authors

  • Antonieta Mengoni (1) Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA-CONICET-UNLP). Calle 47 esq. 116. C.P.1900 La Plata. Buenos Aires. Argentina. (2) Comisión de Investigaciones Científicas (CIC-PBA). La Plata. Buenos Aires. Argentina.
  • Alejandra Viviana Quiroga (1) Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA-CONICET-UNLP). Calle 47 esq. 116. C.P.1900 La Plata. Buenos Aires. Argentina. (3) Facultad de Ciencias Agrarias y Forestales. Universidad Nacional de La Plata. Calle 60 y 119. C.P.1900. La Plata. Buenos Aires. Argentina.
  • María Cristina Añón (1) Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA-CONICET-UNLP). Calle 47 esq. 116. C.P.1900 La Plata. Buenos Aires. Argentina.

DOI:

https://doi.org/10.26461/11.03

Keywords:

antiproliferative activity, Amaranth hypochondriacus lectin

Abstract

Food exerts an important influence on the development of several chronic diseases, including cancer. On the other hand there is evidence indicating that some food components can protect against the development of this disease. The aim of this work was to purify, characterize and analyze the potential antiproliferative activity of a lectin from Amaranthus hypochondriacus.Lectin was partially purified from defatted Amaranthus hipochondriacusflour. A protein with an approximate molecular mass of 65-70 kDa, composed of two subunits of about 35 kDa held together by noncovalent interactions with hemagglutinating activity characteristic of lectins was found. Inhibition of cell proliferation was studied using the tumor cell line CaCo2/TC7 by the crystal violet assay. In addition to the partially purified lectin, the antiproliferative effect of Amaranhus hypochondriacusand mantegazzianusisolates, and a commercial lectin from Amaranthus caudatuswas studied. All samples tested showed cytotoxic effects in a dose dependent way.

Downloads

Download data is not yet available.

Published

2016-07-15

Issue

Section

Articles

How to Cite

Purification and Characterization of a lectin from Amaranthus hypochondriacus, an antiproliferative compound. (2016). INNOTEC, 1(11 ene-jul), 27-35. https://doi.org/10.26461/11.03

Most read articles by the same author(s)