Puesta a punto del método de PCR en tiempo real para la cuantificación de Aspergillus carbonarius en uvas Vitis vinifera cv. Tannat

Authors

  • Gustavo Garmendia Cátedra de Microbiología, Departamento de Biociencias. Facultad de Química, Universidad de la República, Uruguay.
  • Silvana Vero Cátedra de Microbiología, Departamento de Biociencias. Facultad de Química, Universidad de la República, Uruguay.

DOI:

https://doi.org/10.26461/06.10

Keywords:

Ocratoxina A, Sección Nigri, uva

Abstract

Ochratoxin A has been detected in grapes and wines. It is produced by Aspergillus ochraceus, Penicillium verrucosum, As pergillus carbonarius, species belonging to Aspergillus niger aggregate. During the last yars the presence of Aspergillus aculeatus and Aspergillus japonicus in grape surface and its ability to produce OTA has been reported. Our research group isolated, identified and characterized 56 Aspergillus strains; 51 % was identified as A. niger, 39 % as A. japonicus, 5 % as A. foetidus, and 5 % as A. tubingensis like. None A. carbonarius was isolated. This species have been reported as the principal source of OTA in wine grapes and wines due to its ability to produce high mycotoxin concentrations on fruit. Thus, a more sensitive method to quantify A. carbonarius would be important to ensure the absence of this pathogen in grape samples. In this work Real Time PCR for quantification of A. carbonarius in Tannat grapes was optimized.

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How to Cite

Puesta a punto del método de PCR en tiempo real para la cuantificación de Aspergillus carbonarius en uvas Vitis vinifera cv. Tannat. (2011). INNOTEC, 6 ene-dic, 47-50. https://doi.org/10.26461/06.10